Ikram Mercha
Biotechnology / Eco-toxicology & Biodiversity University Hassan II, Morocco
Title: Characterization of technological and probiotic features of interactive lactic acid bacteria isolated from a Moroccan Argane biotope
Biography
Biography: Ikram Mercha
Abstract
Lactic acid bacteria (LAB) occupies a central role as starter cultures in the fermentation process and for their ability to create a healthy equilibrium between beneficial and potentially harmful microorganism in the gut. The objective of this study was to isolate, identify and characterize the technological potential and the probiotic properties of the resident lactic flora in camel milk from a Moroccan Argane biotope. A total of 101 strains of LAB ,obtained from camel milk characterized by a diet based on Argane by-product , were screened for transit tolerance in simulated upper gastrointestinal tract (GI), for adhesion capacity to human intestinal cell lines and for antimicrobial activities against three tested pathogenic strains (E. coli CIP 53126, Staphylococcus aureus and Salmonella enteric CIP 483 CIP 8039). The selected LAB isolates were then identified basing their phenotypic and molecular (16S rRNA) properties. All strains showed good acidifying power, while 95.23% of them can produce texturizing agents (exopolysaccharides) suggesting their possible use to manufacture fermented products. The revelation of the antibacterial activity showed that 61.90% of strains possess an antagonistic effect. Meanwhile, the molecular identification showed that the biodiversity of raw camel milk in the mentioned region is characterized by lactic acid bacteria belonging to the species Lactobacillus Plantarum, Lactobacillus Brevis and Lactococcus lactis sp lactis1. The results obtained indicate interesting technological and probiotic features of isolated lactic strains, belonging to Lactobacillus Plantarum, Lactobacillus Brevis, and Lactococcus lactis sp lactis1 species.